The autohydrolysis of Lentinus edodes was proposed for the extraction of components with
antioxidant properties. Operation under non-isothermal conditions was evaluated and compared
with isothermal heating. The influence of process severity was assessed in the range of 0.18 to 4.89
(temperature between 50 and 250 C), up to 80% (d.b.) The influence of process severity during the
autohydrolysis of Lentinus edodes was assessed in the range 􀀀0.3 to 4.89 (temperature between 50 and
250 C).Upto 80% (d.b.) of the initial raw material could be solubilized at 210 C. The di erent behavior
of the saccharide and phenolic fractions was observed with the treatment temperature. Whereas the
highest concentration of the saccharide components (mainly glucooligosaccharides) was found at
210 C, the maximum phenolic yield was identified at 250 C. The phenolic content and the antiradical
properties of the extract showed a continuous increase with the temperature range studied, and at
250 C, showed antiradical properties comparable to synthetic antioxidants. Autohydrolysis liquid
fractions were used as solvents in the formulation of bioactive starch-based hydrogels, identifying a
positive correlation between the gel softening and the extracts’ bioactivity features.